结核分枝杆菌抗原PE 35和PPE 68基因多态性的初步研究

仇艳; 蒋毅; 刘海灿; 李马超; 万康林

doi:10.3784/j.issn.1003-9961.2015.07.005

结核分枝杆菌抗原PE 35和PPE 68基因多态性的初步研究

doi: 10.3784/j.issn.1003-9961.2015.07.005

仇艳^1,2,3,
蒋毅^1,2,3,
刘海灿^1,2,3,
李马超^1,2,3,
万康林^1,2,3

1.
中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206
2.
2. 感染性疾病诊治协同创新中心, 杭州 310003
3.

基金项目:

国家自然科学基金(No.81401647)

国家科技重大专项(No.2013ZX10003006, 2013ZX10003002-001)

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出版历程
- 收稿日期: 2015-02-11
- 刊出日期: 2015-07-20

Polymorphism of antigens PE 35 and PPE 68 genes of Mycobacterium tuberculosis

QIU Yan^1,2,3,
JIANG Yi^1,2,3,
LIU Hai-can^1,2,3,
LI Ma-chao^1,2,3,
WAN Kang-lin^1,2,3

1.
State Key Laboratory for Communicable Disease Prevention and Control, Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2.
2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China
3.

Funds:

This study were supported by the fund for the Project from National Natural Science Foundation (No.81401647) and the fund for the National Science and Technology Key Project for Priority Communicable Disease Prevention and Control (No.2013ZX10003006,2013ZX10003002-001)

摘要

摘要: 目的为研究结核分枝杆菌RD1区编码PE/PPE蛋白家族中的PE 35和PPE 68基因的多态性,并评估其基因多态性对免疫识别的影响。方法笔者从本实验室保存的来自国内不同地区的2346株结核分枝杆菌复合群临床分离株中,选取代表不同Spoligotyping类型的161 株临床分离结核分枝杆菌菌株,基因扩增后进行序列比对,比较其在T细胞抗原表位的差异。结果在161 株菌株中,23 株显示有PE 35基因多态性,8 株有PPE 68基因多态性。在PE 35基因中,除了菌株JL06018的突变外的其余突变都导致其T细胞抗原表位的改变。PPE 68基因中,62个T细胞表位中有58个(占93.5%)发生了改变。非北京家族PE 35基因的突变率明显高于北京家族 (P0.05),而PPE 68基因突变虽是非北京家族高于北京家族,但差异无统计学意义。结论 PE 35和PPE 68的编码基因存在明显的多态性,并很有可能导致蛋白功能的明显改变。此外,这2个蛋白的人类T细胞表位是高度可变的,提示这2种蛋白可能通过参与分化选择来逃避宿主免疫。北京家族可能比非北京家族更容易被宿主T细胞识别,进而更容易传播。
- 多态性 /
- 结核分枝杆菌 /
- PE 35 /
- PPE 68
Abstract: Objective To understand the polymorphism of antigens PE 35 and PPE 68 genes of Mycobacterium tuberculosis and evaluate the influence of the polymorphism on immune recognition. Methods A total of 161 clinical M. tuberculosis strains isolated in China were selected and comparative sequence analysis of PE 35 and PPE 68 genes was conducted after amplification. Results Among the 161 M. tuberculosis strains, 23 showed the polymorphism in the gene sequence of PE 35 and 8 showed the polymorphisms in the gene sequence of PPE 68. The mutations in all the PE 35 gene, except that in JL06018, influenced the T cell epitopes. In PPE 68 gene, 58 of 62 T cell epitopes changed, accounting for 93.5%. The mutation rate of PE 35 gene in non-Beijing family strains was significantly higher than that in Beijing family strains (P0.05). Although the mutation rate of PPE 68 gene in non-Beijing family strains was higher than that in Beijing family strains, the difference had no statistical significance. Conclusion Polymorphisms were observed in PE 35 and PPE 68 genes, which might resulting in the drastic changes in the functions of these proteins and influence the virulence of the strains. Beijing family strains are more likely to be recognized than non-Beijing family strains by host T cell in PE 35 gene, which facilitates the spread of Beijing family strains.
- Polymorphism /
- Mycobacterium tuberculosis /
- PE 35 /
- PPE 68

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参考文献(25)

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结核分枝杆菌抗原PE 35和PPE 68基因多态性的初步研究

doi: 10.3784/j.issn.1003-9961.2015.07.005

计量

出版历程

Polymorphism of antigens PE 35 and PPE 68 genes of Mycobacterium tuberculosis

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出版历程

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