卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究

李马超 刘海灿 赵秀琴 万康林

李马超, 刘海灿, 赵秀琴, 万康林. 卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究[J]. 疾病监测, 2016, 31(4): 282-287. doi: 10.3784/j.issn.1003-9961.2016.04.006
引用本文: 李马超, 刘海灿, 赵秀琴, 万康林. 卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究[J]. 疾病监测, 2016, 31(4): 282-287. doi: 10.3784/j.issn.1003-9961.2016.04.006
LI Ma-chao, LIU Hai-can, ZHAO Xiu-qin, WAN Kang-lin. Polymorphism of B cell epitopes coded with PE/PPE gene family in BCG[J]. Disease Surveillance, 2016, 31(4): 282-287. doi: 10.3784/j.issn.1003-9961.2016.04.006
Citation: LI Ma-chao, LIU Hai-can, ZHAO Xiu-qin, WAN Kang-lin. Polymorphism of B cell epitopes coded with PE/PPE gene family in BCG[J]. Disease Surveillance, 2016, 31(4): 282-287. doi: 10.3784/j.issn.1003-9961.2016.04.006

卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究

doi: 10.3784/j.issn.1003-9961.2016.04.006
基金项目: 

国家科技重大专项(No.2013ZX10003006-002-001)

Polymorphism of B cell epitopes coded with PE/PPE gene family in BCG

Funds: 

This study was supported by the National Science and Technology Major Project of China (No.2013ZX10003006-002-001)

  • 摘要: 目的 了解13株卡介苗(bacille calmette-gurin, BCG)基因组中由PE/PPE基因家族编码的B细胞抗原表位分布情况, 分析其对BCG免疫保护力的潜在影响。方法 从国际免疫表位数据库(Immune Epitope Data base, IEDB)中检索结核分枝杆菌B细胞抗原表位, 与结核分枝杆菌参考菌株H37Rv的蛋白质组进行序列比对, 确定PE/PPE基因家族编码B细胞抗原表位序列;并与13株BCG基因组进行序列比对, 提取表位编码序列, 分析其在BCG中的分布与变异。结果 6个PE/PPE基因家族的基因编码28个B细胞抗原表位, 21个B细胞抗原表位在13株BCG中高度保守, 7个B细胞抗原表位在BCG中发生基因变异, 变异表位分别产生于BCG的不同形成期。结论 PE/PPE基因家族所表达的蛋白大多为细菌表面蛋白, PE/PPE蛋白家族中B细胞抗原表位的变化可能对BCG的保护力产生影响, 应继续开展基于B细胞表位的BCG遗传特征研究。
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  • 收稿日期:  2015-12-10
  • 刊出日期:  2016-04-20

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