临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析

周妍妍 杨伟明 李世荣 闫东辉 苏建荣

周妍妍, 杨伟明, 李世荣, 闫东辉, 苏建荣. 临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析[J]. 疾病监测, 2016, 31(6): 517-521. doi: 10.3784/j.issn.1003-9961.2016.06.017
引用本文: 周妍妍, 杨伟明, 李世荣, 闫东辉, 苏建荣. 临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析[J]. 疾病监测, 2016, 31(6): 517-521. doi: 10.3784/j.issn.1003-9961.2016.06.017
ZHOU Yan-yan, YANG Wei-ming, LI Shi-rong, YAN Dong-hui, SU Jian-rong. Characteristics of virulence gen distribution and antibiotic resistance of non-O1/O139 Vibrio cholera[J]. Disease Surveillance, 2016, 31(6): 517-521. doi: 10.3784/j.issn.1003-9961.2016.06.017
Citation: ZHOU Yan-yan, YANG Wei-ming, LI Shi-rong, YAN Dong-hui, SU Jian-rong. Characteristics of virulence gen distribution and antibiotic resistance of non-O1/O139 Vibrio cholera[J]. Disease Surveillance, 2016, 31(6): 517-521. doi: 10.3784/j.issn.1003-9961.2016.06.017

临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析

doi: 10.3784/j.issn.1003-9961.2016.06.017
基金项目: 

北京市自然科学基金(No.7164249)

北京友谊医院科研启动基金资助项目(No.yyqdkt2015-23)

Characteristics of virulence gen distribution and antibiotic resistance of non-O1/O139 Vibrio cholera

Funds: 

This study were supported by the fund of program from Beijing Natural Science Foundation (No.7164249) and the program from Beijing friendship hospital, CMU (No.yyqdkt2015-23)

  • 摘要: 目的 了解临床分离的非O1/O139群霍乱弧菌毒力及耐药特征。方法 收集2014-2015年北京友谊医院4-10月肠道门诊分离到的非O1/O139群霍乱弧菌,采用微量肉汤稀释法检测霍乱弧菌对15种抗生素的耐药性;PCR检测霍乱弧菌的毒力相关基因。结果 35株非O1/O139群霍乱弧菌对复方新诺明的耐药率(40.0%)最高,其次是氯霉素(28.5%)和磺胺异恶唑(22.6%),对阿莫西林/克拉维酸、头孢曲松、头孢西丁、头孢吡肟及亚胺培南完全敏感。毒力基因检测显示所有菌株均携带hlyA和ompU,hapA(97.1%)、rtxA(91.4%)、Ⅵ型分泌系统T6SS(94.3%~97.1%)、Ⅲ型分泌系统T3SS(80.0%~85.7%)和nanH(62.9%)阳性率较高;主要的毒力基因型为hlyA-rtxA-hapA-ompU-nanH-vasA-vasK-vasH-vcsC-vcsV-vcsN-vspD(40.0%)。结论 临床分离非O1/O139群霍乱弧菌毒力基因多样化,对抗菌药物耐药性较高,需加强腹泻病例中非O1/O139群霍乱弧菌的毒力及耐药监测。
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出版历程
  • 收稿日期:  2016-01-19
  • 刊出日期:  2016-06-20

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