猪链球菌血清未分型菌株的荚膜多糖基因簇分析

邱小彤 郝琴 白雪梅

邱小彤, 郝琴, 白雪梅. 猪链球菌血清未分型菌株的荚膜多糖基因簇分析[J]. 疾病监测, 2016, 31(11): 925-931. doi: 10.3784/j.issn.1003-9961.2016.11.009
引用本文: 邱小彤, 郝琴, 白雪梅. 猪链球菌血清未分型菌株的荚膜多糖基因簇分析[J]. 疾病监测, 2016, 31(11): 925-931. doi: 10.3784/j.issn.1003-9961.2016.11.009
QIU Xiao-tong, HAO Qin, BAI Xue-mei. Genetic analyzing capsular polysaccharide synthesis gene loci of the non-serotypeable Streptococcus suis[J]. Disease Surveillance, 2016, 31(11): 925-931. doi: 10.3784/j.issn.1003-9961.2016.11.009
Citation: QIU Xiao-tong, HAO Qin, BAI Xue-mei. Genetic analyzing capsular polysaccharide synthesis gene loci of the non-serotypeable Streptococcus suis[J]. Disease Surveillance, 2016, 31(11): 925-931. doi: 10.3784/j.issn.1003-9961.2016.11.009

猪链球菌血清未分型菌株的荚膜多糖基因簇分析

doi: 10.3784/j.issn.1003-9961.2016.11.009

Genetic analyzing capsular polysaccharide synthesis gene loci of the non-serotypeable Streptococcus suis

  • 摘要: 目的 从基因水平上探究猪链球菌菌株血清不可分型的原因及cps基因簇序列变异规律。方法 根据GenBank登记号下载并提取41株猪链球菌血清未分型菌株的cps基因簇序列,根据wzy序列确定菌株的cps型别,并与相应血清型标准菌株的cps基因簇进行序列比对研究。结果 41株血清未分型猪链球菌中,共有37株菌的cps型别属于已知血清型。其中18株与其对应的血清型标准菌株的cps基因簇相比出现部分cps基因的插入、缺失、倒转、替换和移码突变等变异;另有19株与其对应的血清型标准菌株的cps基因簇序列相同或相似,除携带血清24型cps基因簇的菌株LSS23、LSS31、LSS37与血清24型标准菌株88-5299A的cps基因簇同源性为96%外,其余16株与其各自的标准菌株的cps基因簇同源性均超过99%。此外,有4株血清未分型菌株属于已知的新cps型别。结论 由于猪链球菌cps基因簇序列变异频繁,导致荚膜多糖抗原型别呈多样化趋势,传统的血清凝集方法无法有效应对,亟需引入更灵敏的分子血清分型技术开展监测。
  • [1] WertheimHFL,NghiaHDT,TaylorW,etal.Streptococcussuis:anemerginghumanpathogen[J].ClinInfectDis,2009,48(5):617-625.
    [2] YangWZ,YuHJ,JingHQ,etal.AnoutbreakofhumanStreptococcussuisserotype2infectionspresentingwithtoxicshocksyndromeinSichuan,China[J].ChineseJournalofEpidemiology,2006,27(3):185-191.(inChinese)杨维中,余宏杰,景怀琦,等.四川省一起伴中毒性休克综合征的人感染猪链球菌2型暴发[J].中华流行病学杂志,2006,27(3):185-191.
    [3] YeCY,ZhuXP,JingHQ,etal.Streptococcussuissequencetype7outbreak,Sichuan,China[J].EmergInfectDis,2006,12(8):1203-1208.
    [4] YuHJ,JingHQ,ChenZH,etal.HumanStreptococcussuisoutbreak,Sichuan,China[J].EmergInfectDis,2006,12(6):914-920.
    [5] SeguraM,GottschalkM,OlivierM.EncapsulatedStreptococcussuisinhibitsactivationofsignalingpathwaysinvolvedinphagocytosis[J].InfectImmun,2004,72(9):5322-5330.
    [6] Goyette-DesjardinsG,AugerJP,XuJG,etal.Streptococcussuis,animportantpigpathogenandemergingzoonoticagent-anupdateontheworldwidedistributionbasedonserotypingandsequencetyping[J].EmergMicrobesInfect,2014,3(6):e45.
    [7] OkuraM,TakamatsuD,MaruyamaF,etal.GeneticanalysisofcapsularpolysaccharidesynthesisgeneclustersfromallserotypesofStreptococcussuis:potentialmechanismsforgenerationofcapsularvariation[J].ApplEnvironMicrobiol,2013,79(8):2796-2806.
    [8] BaiXM,LiuZJ,JiSB,etal.Simultaneousdetectionof33StreptococcussuisserotypesusingtheluminexxTAGassayTM[J].JMicrobiolMethods,2015,117:95-99.
    [9] LiuZJ,ZhengH,GottschalkM,etal.DevelopmentofmultiplexPCRassaysfortheidentificationofthe33serotypesofStreptococcussuis[J].PLoSOne,2013,8(8):e72070.
    [10] OkuraM,LachanceC,OsakiM,etal.Developmentofatwo-stepmultiplexPCRassayfortypingofcapsularpolysaccharidesynthesisgeneclustersofStreptococcussuis[J].JClinMicrobiol,2014,52(5):1714-1719.
    [11] LiuZJ,BaiXM,JiSB,etal.DevelopmentofamultiplexPCRassaytoidentifysevennewcapsulargenelociofStreptococcussuis[J].ChineseJournalofZoonoses,2014,30(4):337-346.(inChinese)刘志杰,白雪梅,纪少博,等.鉴定7种新型猪链球菌荚膜多糖基因型多重PCR方法的建立[J].中国人兽共患病学报,2014,30(4):337-346.
    [12] KerdsinA,AkedaY,HatrongjitR,etal.StreptococcussuisserotypingbyanewmultiplexPCR[J].JMedMicrobiol,2014,63(6):824-830.
    [13] PanZH,MaJL,DongWY,etal.NovelvariantserotypeofStreptococcussuisisolatedfrompigletswithmeningitis[J].ApplEnvironMicrobiol,2015,81(3):976-985.
    [14] ZhengH,JiSB,LiuZJ,etal.EightnovelcapsularpolysaccharidesynthesisgenelociidentifiedinnontypeableStreptococcussuisIsolates[J].ApplEnvironMicrobiol,2015,81(12):4111-4119.
    [15] WeinertLA,ChaudhuriRR,WangJH,etal.GenomicsignaturesofhumanandanimaldiseaseinthezoonoticpathogenStreptococcussuis[J].NatCommun,2015,6:6740.
    [16] SmithHE,deVriesR,van'tSlotR,etal.ThecpslocusofStreptococcussuisserotype2:geneticdeterminantforthesynthesisofsialicacid[J].MicrobPathog,2000,29(2):127-134.
    [17] CarverTJ,RutherfordKM,BerrimanM,etal.ACT:theArtemiscomparisontool[J].Bioinformatics,2005,21(16):3422-3423.
    [18] MoronaJK,MoronaR,PatonJC.ComparativegeneticsofcapsularpolysaccharidebiosynthesisinStreptococcuspneumoniaetypesbelongingtoserogroup19[J].JBacteriol,1999,181(17):5355-5364.
    [19] YunKW,ChoEY,ChoiEH,etal.Capsularpolysaccharidegenediversityofpneumococcalserotypes6A,6B,6C,and6D[J].IntJMedMicrobiol,2014,304(8):1109-1117.
    [20] LakkitjaroenN,TakamatsuD,OkuraM,etal.Capsulelossordeath:thepositionofmutationsamongcapsulegenesswaysthedestinyofStreptococcussuis[J].FEMSMicrobiolLett,2014,354(1):46-54.
    [21] KnirelYA,WangJP,LuoX,etal.GeneticandstructuralidentificationofanO-acyltransferasegene(oacC)responsibleforthe3/4-O-acetylationonrhamnoseⅢinShigellaflexneriserotype6[J].BMCMicrobiol,2014,14:266.
  • 加载中
计量
  • 文章访问数:  1694
  • HTML全文浏览量:  60
  • PDF下载量:  25
  • 被引次数: 0
出版历程
  • 收稿日期:  2016-08-03
  • 刊出日期:  2016-11-20

目录

    /

    返回文章
    返回

    在线交流

    防诈骗公告

    近期有不法分子以本刊编辑身份添加作者微信,请务必提高警惕!本刊关于稿件的一切事项通知均采用编辑部唯一邮箱(jbjc@icdc.cn)和座机(010-58900732)联系作者,且在录用稿件后仅收取版面费,无其他任何名目费用(如审稿费和加急费等),非编辑部邮箱发送的本刊收费用通知等均为诈骗,不要随意汇入款项!如有可疑及时致电编辑部核实确认!