2014年浙江省湖州市急性腹泻患者诺如病毒感染及基因特征研究

沈月华; 纪蕾; 陈莉萍; 朱晓娟; 查赟峰; 徐德顺; 吴晓芳

doi:10.3784/j.issn.1003-9961.2016.11.012

2014年浙江省湖州市急性腹泻患者诺如病毒感染及基因特征研究

doi: 10.3784/j.issn.1003-9961.2016.11.012

1.
湖州市疾病预防控制中心, 浙江湖州 313000

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出版历程
- 收稿日期: 2016-01-29
- 刊出日期: 2016-11-20

Infection status and genetic diversity of noroviruses in patients with acute gastroenteritis in Huzhou, Zhejiang,2014

1.
Huzhou Prefecture Center for Disease Control and Prevention, Huzhou 313000, Zhejiang, China

摘要

摘要: 目的了解2014年浙江省湖州市急性腹泻患者诺如病毒感染状况及基因特征，为加强感染性腹泻监测与综合防控工作提供依据。方法收集2014年湖州市综合性三级乙等和二级甲等各一家医院的粪便标本797份，采用实时荧光定量PCR方法进行诺如病毒核酸检测，并对诺如病毒核酸阳性标本进行衣壳蛋白区的序列测定和分析。结果 797份粪便标本中149份检出诺如病毒核酸阳性，阳性率18.70%（149/797），以GⅡ基因型为主，占89.26%（133/149）。衣壳蛋白区序列的系统进化分析显示检出的诺如病毒有5种GⅠ型别和7种GⅡ型别，包括GⅠ.1、GⅠ.2、GⅠ.5、GⅠ.6、GⅠ.7、GⅡ.2、GⅡ.3、GⅡ.4、GⅡ.6、GⅡ.8、GⅡ.17、GⅡ.21，以GⅡ.4 Sydney_2012（55.5%）、GⅡ.17（25.5%）型为主。结论诺如病毒在湖州市急性腹泻患者中存在较高感染，并且基因型别复杂，除了流行优势型别GⅡ.4外，2014年底还出现GⅡ.17型诺如病毒的流行，因此应加强诺如病毒腹泻监测工作，为今后疫情防控提供科学依据。
- 诺如病毒 /
- 腹泻 /
- 基因分型
Abstract: Objective To understand the infection status of norovirus in patients with acute gastroenteritis in Huzhou, Zhejiang province, in 2014, and the genetic characteristics of norovirus isolated from the patients. Methods A total of 797 stool samples were collected from outpatients with acute gastroenteritis in 2 hospitals in Huzhou from January to December 2014. Real-time RT-PCR was performed to detect norovirus nucleic acid, the positive samples were used for the sequencing of capsid protein of norovirus. Results Among the 797 stool samples, 149 were norovirus positive (18.70%), and norovirus GⅡ was predominant, accounting for 89.26% (134/149). At least 12 genotypes were identified among GⅠ and GⅡ isolates, including GⅠ.1, GⅠ.2, GⅠ.5, GⅠ.6, GⅠ.7, GⅡ.2, GⅡ.3, GⅡ.4, GⅡ.6,GⅡ.8, GⅡ.17 and GⅡ.21. GⅡ.4 Sydney_2012 (55.5%) was the most frequently detected genotype, followed by GⅡ.17 (25.5%). Conclusion Our findings suggested that norovirus was the common causative agent of acute gastroenteritis in Huzhou during the study period, the genetic characteristics of the norovirus circulating in Huzhou were complex. Except predominant GⅡ.4 Sydney_2012, the infection caused by GⅡ.17 strains occurred in late 2014, suggesting the importance of strengthened surveillance for diarrhea caused by norovirus in Huzhou for the future epidemic prevention and control.
- Norovirus /
- Gastroenteritis /
- Genotype

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参考文献(14)

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文章访问数: 1600
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2014年浙江省湖州市急性腹泻患者诺如病毒感染及基因特征研究

doi: 10.3784/j.issn.1003-9961.2016.11.012

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出版历程

Infection status and genetic diversity of noroviruses in patients with acute gastroenteritis in Huzhou, Zhejiang,2014

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