云南省不明原因发热患者恙虫病东方体实验室检测分析

亚红祥 张云智

亚红祥, 张云智. 云南省不明原因发热患者恙虫病东方体实验室检测分析[J]. 疾病监测, 2017, 32(6): 500-503. doi: 10.3784/j.issn.1003-9961.2017.06.014
引用本文: 亚红祥, 张云智. 云南省不明原因发热患者恙虫病东方体实验室检测分析[J]. 疾病监测, 2017, 32(6): 500-503. doi: 10.3784/j.issn.1003-9961.2017.06.014
YA Hong-xiang, ZHANG Yun-zhi. Laboratory detection of Orientia tsutsugamushi in patients with fever of unknown origin in Yunnan province,China[J]. Disease Surveillance, 2017, 32(6): 500-503. doi: 10.3784/j.issn.1003-9961.2017.06.014
Citation: YA Hong-xiang, ZHANG Yun-zhi. Laboratory detection of Orientia tsutsugamushi in patients with fever of unknown origin in Yunnan province,China[J]. Disease Surveillance, 2017, 32(6): 500-503. doi: 10.3784/j.issn.1003-9961.2017.06.014

云南省不明原因发热患者恙虫病东方体实验室检测分析

doi: 10.3784/j.issn.1003-9961.2017.06.014

Laboratory detection of Orientia tsutsugamushi in patients with fever of unknown origin in Yunnan province,China

  • 摘要: 目的 对云南省不明原因发热患者进行恙虫病东方体检测与分析。方法 应用间接免疫荧光方法(IFA)和PCR(nPCR)法对不明原因发热患者分别进行恙虫病东方体(Ot) IgG抗体和sta56基因检测及其基因序列测定分析。结果 在79例发热患者中, Ot IgG抗体阳性率48.10%, Ot核酸阳性率3.80%。基因序列分析显示序列12-17与日本O3株同源性最高为99.8%,序列12-9与中国台湾KM05、TW45R等株的同源性最高均为99.8%,序列11-6与中国台湾KM05、TW45R等株的同源性最高均为100%。进化树分析显示序列12-17、12-9和11-6均为Karp型Ot。结论 云南省恙虫病患者中存在Karp型Ot感染,其基因进化来源可能复杂。
  • [1] TamuraA,OhashiN,UrakamiH,etal.ClassificationofRickettsiatsutsugamushiinanewgenus,Orientiagen.nov.,asOrientiatsutsugamushicomb.nov.[J].IntJSystBacteriol,1995,45(3):589-591.
    [2] KohGCKW,MaudeRJ,ParisDH,etal.Diagnosisofscrubtyphus[J].AmJTropMedHyg,2010,82(3):368-370.
    [3] CraccoC,DelafosseC,BarilL,etal.Multipleorganfailurecomplicatingprobablescrubtyphus[J].ClinInfectDis,2000,31(1):191-192.
    [4] KellyDJ,FuerstPA,ChingWM,etal.Scrubtyphus:thegeographicdistributionofphenotypicandgenotypicvariantsofOrientiatsutsugamushi[J].ClinInfectDis,2009,48Suppl3:S203-230.
    [5] ZhangWY,WangLY,DingF,etal.ScrubtyphusinmainlandChina,2006-2012:theneedfortargetedpublichealthinterventions[J].PLoSNeglTropDis,2013,7(12):e2493.
    [6] ZhangM,WangXJ,ZhaoZT.Currentepidemicstatusandissuesonpreventionandcontrolofscrubtyphus[J].ChineseJournalofEpidemiology,2011,32(4):419-423.(inChinese)张萌,王显军,赵仲堂.中国恙虫病流行态势及预防控制[J].中华流行病学杂志,2011,32(4):419-423.
    [7] YunnanPreventiveMedicineAssociation,YunnanProvincialCenterforDiseaseControlandPrevention.Yunnanshengzhongdianchuanranbingfangzhizhinan[M].Kunming:YunnanScienceTechnologyPress,2004:289-433.(inChinese)云南省预防医学会,云南省疾病预防控制中心.云南省重点传染病防治指南[M].昆明:云南科技出版社,2004:289-433.
    [8] XuanQ,LiuAH,BaoFK.CurrentprogressinresearchoftsutsugamushidiseaseinYunnanprovinceinChina[J].ChinaTropicalMedicine,2010,10(10):1278-1280.(inChinese)宣群,柳爱华,宝福凯.云南恙虫病研究概况[J].中国热带医学,2010,10(10):1278-1280.
    [9] ChineseCenterforDiseaseControlandPrevention.Tsutsugamushidiseasepreventionandcontroltechnicalmanual(Pilotedition)[Z].Beijing:ChineseCenterforDiseaseControlandPrevention,2009.(inChinese)中国疾病预防控制中心.恙虫病预防控制技术指南(试行)[Z].北京:中国疾病预防控制中心,2009.
    [10] ReeHI,KimTE,LeeIY,etal.DeterminationandgeographicaldistributionofOrientiatsutsugamushiserotypesinKoreabynestedpolymerasechainreaction[J].AmJTropMedHyg,2001,65(5):528-534.
    [11] YaHX,ZhangHL,ZhouJH,etal.AnoutbreakofendemictyphusinBaoshancity,Yunnanprovince[J].ChineseJournalofEpidemiology,2011,32(1):47-50.(inChinese)亚红祥,张海林,周济华,等.云南省保山市一起地方性斑疹伤寒暴发的调查[J].中华流行病学杂志,2011,32(1):47-50.
    [12] YaHX,WangJL.ThedetectionofspottedfevergrouprickettsiaDNAfromfeverpatientsinDali,Yunnan[J].DiseaseSurveillance,2012,27(6):432-434.(inChinese)亚红祥,王静林.云南省大理州发热患者血液中检测斑点热群立克次体[J].疾病监测,2012,27(6):432-434.
    [13] XieZG,XuYH,OuyangN.FirstoutbreakoftsutsugamushidiseaseinDongchuandistrictofKunmingcity[J].ChineseJournalofEpidemiology,2003,24(7):627.(inChinese)谢志刚,徐月恒,欧阳宁.昆明市东川区首次恙虫病爆发[J].中华流行病学杂志,2003,24(7):627.
    [14] WangQX,LiYM,LiuJZ.AnoutbreakofendemictyphusfromavillageofDali,Yunnanprovince[J].ModernPreventiveMedicine,2006,33(6):1010.(inChinese)王庆新,李育民,刘继政.某村地方性斑疹伤寒暴发的调查[J].现代预防医学,2006,33(6):1010.
    [15] LiL.29casesoftsutsugamushidiseasefromaPrimaryschool[J].PracticalClinicalMedicine,2006,7(3):150.(inChinese)李丽.小学生恙虫病29例集体发病临床分析[J].实用临床医学,2006,7(3):150.
    [16] YaHX,ZhangHL.Detectionofantibodyagainstscrubtyphusinserafrompatientsinanoutbreakofmurinetyphus[J].JournalofTropicalDiseasesandParasitology,2014,12(3):161-162.(inChinese)亚红祥,张海林.一起鼠型斑疹伤寒暴发疫情中患者血清恙虫病抗体检测[J].热带病与寄生虫学,2014,12(3):161-162.
    [17] ZhangYB,ZhangLY,ChenLX.InvestigationonanoutbreakofscrubtyphusinEshancounty,Yunnanprovince[J].MedicineandPharmacyofYunnan,2014,35(1):64-65.(inChinese)张玉宝,张丽英,陈利新.峨山县一起恙虫病暴发的调查报告[J].云南医药,2014,35(1):64-65.
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  • 收稿日期:  2017-01-23
  • 刊出日期:  2017-06-20

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