Abstract: Objective To understasnd the molecular epidemiological characteristics of Salmonella typhi in Xinjiang between 1995 and 2016,and provide evidence for the disease surveillance and epidemic early warning. Methods Pulsed field gel electrophoresis(PFGE)was used to subtype 527 S. typhi strains isolated in seven prefectures in Xinjiang between 1995 and 2016. Results A total of 527 S. typhi strains were divided into 145 PFGE types,and the similarity was 57.42%-100.00%. Some PFGE types have persisted for many years and were detected in different areas. A total of 32 groups of cluster cases were found. Conclusion S. typhi isolates in Xinjiang are highly polymorphic in genotype,and there is a long-term existence of predominant types. There is epidemiological clustering of cases indicated by molecular typing. It is necessary to strengthen the laboratory surveillance.