甘肃省53株非结核分枝杆菌对9种常用抗结核药物的敏感性分析

蔡静 张鑫 魏黛珏 李凯 同重湘

蔡静, 张鑫, 魏黛珏, 李凯, 同重湘. 甘肃省53株非结核分枝杆菌对9种常用抗结核药物的敏感性分析[J]. 疾病监测, 2018, 33(6): 515-519. doi: 10.3784/j.issn.1003-9961.2018.06.016
引用本文: 蔡静, 张鑫, 魏黛珏, 李凯, 同重湘. 甘肃省53株非结核分枝杆菌对9种常用抗结核药物的敏感性分析[J]. 疾病监测, 2018, 33(6): 515-519. doi: 10.3784/j.issn.1003-9961.2018.06.016
Cai Jing, Zhang Xin, Wei Daijue, Li Kai, Tong Chongxiang. Analysis on susceptibility of 53 non tuberculosis mycobacterium isolates to 9 common anti-tuberculosis drugs in Gansu[J]. Disease Surveillance, 2018, 33(6): 515-519. doi: 10.3784/j.issn.1003-9961.2018.06.016
Citation: Cai Jing, Zhang Xin, Wei Daijue, Li Kai, Tong Chongxiang. Analysis on susceptibility of 53 non tuberculosis mycobacterium isolates to 9 common anti-tuberculosis drugs in Gansu[J]. Disease Surveillance, 2018, 33(6): 515-519. doi: 10.3784/j.issn.1003-9961.2018.06.016

甘肃省53株非结核分枝杆菌对9种常用抗结核药物的敏感性分析

doi: 10.3784/j.issn.1003-9961.2018.06.016
基金项目: 

兰州市科技计划项目(No.2014-ZD-01)

详细信息
    作者简介:

    蔡静,女,甘肃省兰州市人,主管检验师,主要从事微生物的分离鉴定和新病原的发现

  • 中图分类号: R378

Analysis on susceptibility of 53 non tuberculosis mycobacterium isolates to 9 common anti-tuberculosis drugs in Gansu

Funds: 

This study was supported by the fund for the Scientific Research Project of Lanzhou Municipality(No. 2014-ZD-01)

  • 摘要: 目的 初步了解2012-2014年甘肃省临床分离的非结核分枝杆菌(NTM)对常用抗结核药物的耐药情况。方法 收集临床分枝杆菌分离株,用PNB/TCH生长试验和基于16S rRNA基因的序列分析法鉴定NTM。采用传统固体罗氏培养基绝对浓度法对NTM进行9种常用抗结核药物链霉素(SM)、利福平(RFP)、丙硫异烟胺(TH1321)、异烟肼(INH)、对氨基水杨酸钠(PAS)、左氧氟沙星(LVFX)、乙胺丁醇(EMB)、卡那霉素(KAN)和吡嗪酰胺(PZA)的敏感性测试。结果 共收集到分枝杆菌1 081株,经鉴定53株为NTM,分离率为4.90%。其中胞内分枝杆菌最多,为36株;其次是堪萨斯分枝杆菌,为8株;其他依次是鸟分枝杆菌4株、戈登分枝杆菌2株、脓肿分枝杆菌2株和偶然分枝杆菌1株。药敏结果显示,耐药率从高到低依次为RFP(73.58%)、TH1321(69.81%)、INH(67.92%)、SM(62.26%)、PAS(60.38%)、PZA(47.17%)、EMB(28.30%)、LVFX(26.41%)、KAN(15.09%),其中耐药株49株,耐药率为92.45%,NTM对9种抗结核药物的耐药程度有差异。结论 NTM对抗结核药物敏感性差,应参照NTM药敏结果选择合理的药物,提高治愈率,减少耐药发生。
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