Abstract: Objective To understand the epidemiology characteristics of human infection with avian influenza A (H7N9) virus in Huaiji county, Guangdong province and evaluate the influence of suspension of live poultry trade on the epidemic. Methods Descriptive epidemiological analysis was conducted on the incidence data of human infection with avian influenza A (H7N9) virus in Huaiji. Environmental samples were taken from the domestic poultry raising sites of the 9 cases and live poultry markets before and after closure to detect the nucleic acid of H7N9 virus with real-time PCR. Results A total of 9 cases of human infection with H7N9 virus were confirmed in Huaiji, no death case was reported. Severe pneumonia developed in severe cases and mild cases usually had cough and sore throat. The cases were distributed in five townships and the median age of the cases was 11 years (4-62 years).All the cases had exposures to poultry and 4 had chronic disease. Six of 164 close contacts of the 9 cases had influenza like symptoms, and 1 was confirmed to be infected with H7N9 virus, given the same source exposure. The H7 antibody positive rate of environment samples was 65.00% (52/80) in H townships and 14.81% (12/81) in other townships before the exposure of live poultry markets, but the H7 antibody positive rate of environmental samples was 3.49% (3/86) in H township and 0 (0/111) in other townships after the closure of live poultry markets. The case number of human infection with H7N9 virus declined obviously after the closure of live poultry markets in Huaiji. Conclusion All the 9 cases confirmed had mild infections with H7N9 virus, and children aged 4-11 years were mainly affected. All the cases had live poultry exposure histories. Comprehensive prevention and control measures, including the suspension of live poultry markets, could effective control the epidemic.