中国蜡样芽胞杆菌脉冲场凝胶电泳标准化技术的建立与应用

Establishment and application of standard pulsed-field gel electrophoresis assay to detect Bacillus cereus

摘要: 目的本研究旨在选择不同的内切酶和电泳条件对蜡样芽胞杆菌(蜡样杆菌)脉冲场凝胶电泳(PFGE)方法进行评价,建立中国蜡样杆菌标准PFGE方法. 方法蜡样ATCC国际标准菌株及分离自我国不同地区和来源的蜡样杆菌,制备胶块用溶菌酶和蛋白酶K分别裂解后,选用不同的内切酶对胶块内DNA进行酶切,比较PFGE电泳结果的分离度,评价获得最佳操作方案,选取51株蜡样杆菌菌株进行PFGE实验,对此实验方法进行评价分析. 结果确立的蜡样杆菌 PFGE 分型方法的标准操作程序,可以较好地对蜡样杆菌进行分子分型研究,初步描述了51株蜡样杆菌菌株PFGE型别特征. 结论实验确定的蜡样杆菌PFGE方法,可以为蜡样杆菌引起的食源性疾病及医院感染的溯源控制提供技术手段.

Abstract: Objective To establish a standard pulsed-field gel electrophoresis (PFGE) assay for the detection of Bacillus cereus. Methods Fifty-one B. cereus strains isolated from different source were selected to evaluate the discriminatory power of the established PFGE assay. Results The established PFGE assay for the detection of B. cereus successfully identified the genotypes of 51 strains of B.cereus. Conclusion The PFGE assay was established for the genetic classification of B. cereus which can provide technical support for the control and prevention of food poisoning and nosocomial infection caused by B. cereus.